Abstract
Paenibacillus sonchi LMG 24727T was acquired from the Belgian Coordinated Collections of Microorganisms (BCCM) isolated from Sonchus oleraceus rhizosphere soil. This strain is a gram-positive, aerobic, rod-shaped bacterium. The strain’s genomic DNA was extracted using a Wizard® Genomic DNA Purification Kit, and whole-genome sequencing was performed using the Nanopore MinION platform. Whole-genome assembly was performed using Flye assembler, and a total 7,782,254 bp length of circular chromosome and two plasmids was assembled by using a 1,558,445,868 bp length of raw reads. Genome annotation by the Prokaryotic Genome Annotation Pipeline (PGAP) showed the complete genome to contain 50.6% G+C content; 6264 protein-coding genes; 27 rRNA genes; 89 tRNA genes; and 4 ncRNA genes. Additionally, multiple genes related to nitrogen metabolism were annotated from the Rapid Annotation using Subsystem Technology (RAST) server. The complete genome sequence data have been submitted to the National Center for Biotechnology Information (NCBI) and have been deposited at DDBJ/ENA/GenBank under the accession number CP068595.1, CP068596.1, and CP068597.1.
Min-Ji Kim
Ph.D candidate of Applied Biosciences
My research interests include metagenomics in one-health approach and host-micrbe interactions.